LFT/Molecular Crossover.21 Oct 2024 23:08
The panacea for a POC diagnostic is that it meets the WHO REASSURED criteria;
REal--time connectivity.
Affordable
Sensitive
Specific
User friendly
Rapid equipment free
Delivered
The downfall for LFT’s has been sensitivity and specificity. They meet all other criteria, hence the drive to improve performance. In this respect ABDX have spent loads of time over the last 24 months presenting at conferences and doing workshops (with Cytiva) on the LFT/Molecular crossover - in other words a test with all the normal benefits of an LFT but with performance characteristics akin to PCR, LAMP etc.
Some might think that’s way off, but that’s just not true. By way of example, here’s a paper from UCL back in 2021 where they developed just such a test for Covid.. I don’t understand all the scientific stuff, but I can understand the conclusion;
“ we have developed an ultrasensitive and specific multi-gene diagnostic for SARS-CoV-2 viral RNA using isothermal RPA technology, and proposed two different detection methods, both showing high accuracy. While real-time fluorescence detection developed here offers more sensitivity and faster results (10 min faster than dipstick method), the proposed detection on dipsticks appeared as the preferred method for decentralised testing. We showed this method has the potential to meet the ASSURED and REASSURED criteria; it is affordable, rapid, has high analytical sensitivity and specificity, it is user-friendly and can be performed with minimal equipment. We also proposed the addition of real-time connectivity through a smartphone application and the potential use of saliva as a non-invasive specimen”
As you can see, the “dipstick” method was preferred. Interesting then that “The PCRD FLEX dipsticks and running buffer were obtained from Abingdon Health.”
Remember, this was from over 3 years ago. Plenty of R&D since then. We often forget about our PCRD product but it’s at the very heart of a lot of innovation at the moment.
https://pmc.ncbi.nlm.nih.gov/articles/PMC8120794/
Oh, and whilst the dipstick method was preferred, the other method used stuff provided by Axxin (that company we’ve been working with for a while to integrate PCRD into their tools.