Mygo5 Feb 2022 14:46
Tks to ti y up down appears to be 2022 although it is referred to as ireland.
4.7. Quantitative Real-Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR)
After treatment with propolin G, total RNA was collected by the RNA mini kit (Qiagen, Taipei, Taiwan), and the cDNAs were synthetized by commercial cDNA reverse transcription kit (Invitrogen, Taipei, Taiwan). qRT-PCR was conducted by PrecisionPLUS qPCR Master Mix premixed with SYBRgreen (Primerdesign, Camberley, UK) on MyGo Pro RT-PCR system (IT-IS Life Science, Dublin, Ireland). The primer sequences of real- time PCR are shown below: E-cadherin Forward: 5'-TCGGCCTGAAGTGACTCGTA-3',
Full report
https://mdpi-res.com/d_attachment/ijms/ijms-23-01672/article_deploy/ijms-23-01672.pdf