RE: Cellserch old technology15 Jan 2024 15:21
The Cellsearch System is, I believe, the most commonly CTC capture method used, however it has limitations.
My understanding is it is because of the frequent loss of epithelial antigens by CTC assays targeting epithelial antigens it may miss what can be the most invasive cell population. There is a paper on this by A Gradilone
A more recent review of biomarkers highlights some of the issues of using CellSearch-
'.....pancreatic ductal adenocarcinoma (PDAC), giving a result of only 20% even in the advanced metastatic stage . This contributes to the underestimation of CTC number and could be, in part, due to the high number of CTCs with a mesenchymal phenotype undergoing EMT (i.e., from hybrid CTCs to EMT-CTCs).
Similarly, ' a study conducted on malignant pleural mesothelioma using the CellSearch® system showed a low detection rate of 33%, suggesting a need for efficient EpCAM-independent detection systems of CTCs.'
It is a limitation they mention is the assay details, along with a need to make sure samples are clear of Dox.
Note EMT=epithelial-mesenchymal transition