RE: Saliva Trials results are out!22 Jul 2020 10:39
RNA extraction:
The saliva sample collected within the HHFT was extracted using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega UK Ltd., Southampton, UK) according to manufacturer's instructions. Briefly, 200 µl of sample was added to 223 µl of prepared lysis solution (including 5 µl per reaction of Genesig® Easy RNA Internal extraction control, Primerdesign Ltd, Chandler's Ford, UK)
Acid Purification Kit (Promega UK Ltd., Southampton, UK) according to manufacturer's instructions. 132
Briefly, 200 µl of sample was added to 223 µl of prepared lysis solution (including 5 µl per reaction of 133
Genesig® Easy RNA Internal extraction control, Primerdesign Ltd, Chandler's Ford, UK)