Ryan Mee, CEO of Fulcrum Metals, reviews FY23 and progress on the Gold Tailings Hub in Canada. Watch the video here.
It was a no warning departure. Rough.
No idea if they’re appointing someone else.
Not sure.
Guess all will be revealed soon. Strange timing.
Official now then.
Amrik gone.
Ebbs. Was that from Katrina? I got an email from her about some protein purification dialysis tubes and she used “E Mail” in mine too. Never seen anyone write it like that.
Anyway, they seem busy/disorganised (delete as appropriate).
Apre: Where did you get £120,000 Euros from? It’s £113 for 25ug.
And that is quite a lot of material. You have to take into account the size of the protein (affimer) you are buying. Affimers are small (compared to an antibody) at 16kDa (IgGs are about 150kDa), so when working out the molarity, you take this into account. Ie in 25ug of either affimer or IgG, the number of molecules of affimer will be 10x higher (approx) than the IgG.
E.g. if you bought that 25ug, you’d either receive it as a liquid or as a tiny spec of powder in a tube (in this case you’d resuspend it in water, as the buffer contains pbs). I can’t tell without a CoA how they give it to you, it says ships on dry ice, so I guess liquid that is frozen and then the first time you use it you thaw and aliquot smaller amounts.
Say they give to you at 100ul. That is 250ug/mL. The size is 16kDa.
(My brain normally hurts doing these calculations. I just remember 1mg/ml for IgG is /0.15 (150kDa) = 6.66uM, or if you divide by 150,000 it’s 0.0000067M.)
So for this: 250ug/ml = 0.25mg/ml
0.25/0.016 (16kDa) = 15.6uM.
As the affinity (kD) is 20pM (very high affinity), you are way above that at 15.6uM. (About 1 million fold). That’s why it says do a 1:1000 dilution, even this is probably wasting a lot of your material!
Hope this makes some sense. TLDR: a ug of something is not always tiny!
Research. Other biotech/academia/pharma.
*with AffiDx
Do they regularly test their own employees?
Sorry this is a bit of a post fail. My last comment was less to do with Calibre/Bioserv, and more to do with your recent LinkedIn research.
You live up to your name! Guess it’s easy to find information these days. Keep an eye on LinkedIn in August.
Our* Sepharose* sorry still haven’t woken up.
Huh? I woke up early this morning and I see a post about Paul Driver? So I thought I’d comment. Jeesh. We got out Protein A Sepahrose beads in the end at a decent price, about 10% cheaper than Generon (where Paul used to work I think). Just thought it was a funny coincidence. I think I remember reading about Calibre being a distribution partner but the name hadn’t clicked as I was dealing with ProteinArk back then.
Omg. That guy! I was trying to place an order for protein purification stuff from ProteinArk in early May. Sent this guy email after email asking to add stuff to our quote, never heard a thing. Assumed he had left the company or was dead. Put our order in via a generic email address in the end.
I didn’t say it was a terrible company. My friend works incredibly hard. Harder than me, clearly! Back to work for me!
Was just pointing out that Biotech/Pharma CEOs like to exaggerate, or take the best figure. For example if I tell my boss we can express antibodies at 50 to 200mg/L, then he’ll tell clients we can do 200. I guess it’s just business, but often it causes issues, or unrealistic expectations.
It’s funny you think I’d lie?
I work in Cambridge for Biotechs. Have done for nearly a decade. I’ve walked past whittlesford parkway train station more times than I care to remember.
Good luck with the investing.
I don’t really understand this language. I read this board from time to time, as I know someone who works at Avacta and I like sending them screengrabs. My personal highlight was:
“AS may be learning some harsh lessons about the protracted authorisation procedures required in the development of any diagnostic/therapeutic device/medicinal agent and he's had the additional frustration with the LFTs of bumbling political/bureaucratic issues. His vision is clearly huge and essentially unstoppable. I take great comfort in that vision, intelligence and energy. These are very rare qualities when rolled into one individual.”
We had a good laugh.
It must be a weird feeling hoping covid will go on and on, just to make a few quid.
Israel’s vaccination programme seemed to hit a wall at 60% of their population vaccinated. Who are the 40%? Don’t think children account for that.