Primer design kit14 Mar 2020 10:01
The genesig Real-Time PCR BKV Kit (CE) is intended to be used on the Applied Biosystems 7500 Real-Time PCR System, Bio-Rad CFX Real-Time PCR Detection System, and Roche LightCycler 480 Instrument II, to achieve quantitative detection of BKV DNA extracted from blood plasma and urine from immunocompromised patients in association with the designated extraction instrument and kits:
Automated extraction - Roche MagNA Pure 24 and Roche MagNA Pure 24 Total NA Isolation Kit (for plasma and urine samples)
Manual extraction - QIAGEN QIAamp® DNA Mini Kit (for plasma samples), or QIAGEN QIAamp® Viral RNA Mini Kit (for urine samples)
Quantification Standards in the kits are calibrated against the First World Health Organization International Standards for Virus Nucleic Acid Amplification Techniques (NIBSC code 14/212), and demonstrate the kit has strong levels of sensitivity and specificity performance, as well as allowing harmonisation of results across labs.