RE: PARAYTEC WORLDWIDE PATENTS7 Apr 2021 16:08
FULL DESCRIPTION OF DEVICE
https://worldwide.espacenet.com/publicationDetails/description?CC=US&NR=2005231718A1&KC=A1&FT=D&ND=4&date=20051020&DB=&locale=en_EP
[0001] The present invention relates to an optical assembly comprising a sample vessel positioned in a direct light path between a light source and a light detector, in manner to enable transmission of light through the vessel; a method for detection of light transmission through sample contained within the vessel; an apparatus comprising the assembly; more particularly an apparatus for sample analysis for example for high throughput screening (HTS) or profiling or assays, such as enzyme assays; and uses thereof in the pharmaceutical, biomedical and bioscience, agrochemical, veterinary, materials and like fields, for detection, analysis, characterisation and quantification or the like of samples contained in a vessel, and optionally further collecting separated components thereof; in particular in combinatorial chemistry; in metabolomics, proteomics or genomics, assay and high throughput analysis applications, typically high sensitivity analyses, separation and/or quantification studies and for sample separation for example chromatography or electrophoresis, in particular column chromatography, capillary electrophoresis with real time or post separation analysis.
[0002] UV absorbance, fluorescence and mass spectrometry are key technologies used in separation science for analysing species in samples. A particularly useful methodology is to look at a sample population separated by capillary electrophoresis with fluorophore labelling and fluorescence imaging, for quantification, and MS for characterising molecules of interest.
[0003] U.S. Pat. No. 5,582,705 discloses an apparatus and system for laser induced fluorescence (LIF) detection in a multiplexed capillary electrophoresis system. A coherent beam incident on the capillary array and emitted fluorescent light are typically perpendicular to each other in order to reduce background noise due to light scattering. A transparent portion in each capillary wall defines a transparent path extending through the array, perpendicular to the capillary. A 2D image array detector such as a charge-coupled device (CCD), preferably a charge-injection device (CID), is positioned to detect emission, and an imaging lens interposed between the capillary array and the image array detector, to optically couple the pixels to the capillary. The imaging lens may be any lens capable of transforming an image onto the pixels of the image array detector, such as a camera lens or a condenser lens. Coupling is shown in FIG. 4, of U.S. Pat. No. 5,582,705 in which every second pixel is coupled to a sidewall of the capillary and every pixel in between is coupled to an interior portion.