Portfolio Update

HENDERSON INVESTMENT FUNDS LIMITED

HENDERSON ALTERNATIVE STRATEGIES TRUST PLC

LEGAL ENTITY IDENTIFIER: 213800J6LLOCA3CUDF69

2 April 2019

HENDERSON ALTERNATIVE STRATEGIES TRUST PLC

Portfolio update

In accordance with Listing Rule 15.6.8 the Company discloses that as at 31 March 2019 it held the following investments in other listed closed-ended investment funds which do not have a stated investment policy to invest no more than 15% of their total assets in other listed closed-ended investment funds:

Holding % of the Company's gross assets

3i Group plc 2.10

Axiom European Financial Debt Fund Ltd 2.19

Worldwide Healthcare Trust plc 3.11

The Company uses data from the Association of Investment Companies (the "AIC") to identify whether the Company's investments in other listed closed-ended investment funds do not have a stated investment policy.

The Company has been made aware that one of its previously held positions in the listed closed-end investment fund 3i Group plc had not been correctly categorised by the AIC.

The AIC has confirmed that this has now been amended.

For further information, please call:

Colleen Sutcliffe

For and on behalf of Henderson Secretarial Services Limited

Secretary to Henderson Alternative Strategies Trust plc

Telephone: 020 7818 5919

Laura Thomas

Investment Trust PR Manager

Janus Henderson Investors

Telephone: 020 7818 2636