Monthly Factsheet as at 31 August 2020

HENDERSON ALTERNATIVE STRATEGIES TRUST PLC

HENDERSON INVESTMENT FUNDS LTD

LEGAL ENTITY IDENTIFIER: 213800J6LLOCA3CUDF69

28 September 2020

HENDERSON ALTERNATIVE STRATEGIES TRUST PLC

Monthly Factsheet as at 31 August 2020

A copy of the above document has been submitted to the National Storage Mechanism and will shortly be available for inspection at: https://data.fca.org.uk/#/nsm/nationalstoragemechanism.

The Factsheet contains information on the progress of the Company's investment realisation. Further context on this and our approach to future disclosure can be found in the factsheet.

Cash as a percentage of NAV (as at 18 September) stood at 62.3%, or approximately £77.9m.

Details of the full realisations for the period 22 July 2020 to 14 August 2020 are available in the September factsheet.

The factsheet will shortly be available on the Company's website, www.hendersonalternativestrategies.com/factsheet.

For further information please contact:

Laura ThomasInvestment Trust PR ManagerJanus Henderson InvestorsTelephone: 020 7818 2636

Neither the contents of the Company's website nor the contents of any website accessible from hyperlinks on the Company's website (or any other website) is incorporated into, or forms part of, this announcement.