RE: Covid-19 mutations. Scary.27 Apr 2020 13:12
Primers are designed to amply the RNA which allow for detection of mutations provided they are not within the forward and back primer which are very small nucleotide sequences and so the overwhelming majority of the 30 mutations will be detected using this technique as they won't be in this 10 nucleotide sequence. I would speculate and say over 95-99% will be detected especially when lengths can be from 9,000 to 29,000 nucleotides in length!!