Member Info for Ophidian

you know some of you are very rude. I invested a little time and knowledge to help answer a question on here that had vexed the board for days without a proper answer and for my trouble there is this Tsunami of bad blood about another share I'm probably most associated with which I have never even mentioned over here. This board needs to grow up a bit. You won't be getting further input from me now that is for damn sure. Good luck with your investments.

Ophidian

after this evenings discussion of LOD I've done a little digging. The Zika Affimer test detects "early" viral loads and from various sources the peak load is an average of 9.9x 10^4 or 99,000 copies per ml this implies that the Affimer detects less than 99,000 as that is pre peak. From the Lancet article on Hong Kong patients,

" The median viral load in posterior oropharyngeal saliva or other respiratory specimens at presentation was 5·2 log10 copies per mL (IQR 4·1–7·0). Salivary viral load was highest during the first week after symptom onset and subsequently declined with time (slope -0·15, 95% CI -0·19 to -0·11; R2=0·71). "

5.2log10 copies per ml is 158,000 copies per ml. So I would say that at onset of symptoms and probably well before the affimer concentration on the strip is more than enough to detect the virus based on Zika.

Ophidian

girlupnorth - not wanting to split hairs but so anyone else reading this and future information may differentiate appropriately. Since this is a Binary test - the strip turns blue or it doesn't. This is not a question of sensitivity which is defined as the ability of a method to differentiate between different but close concentrations - this is a question of establishing what is the Limit of detection (LOD). The sensitivity is generally referred to as the being the slope of the calibration curve where as the LOD is the intercept.

I am also unaware of any published information pertaining to the LOD's for affimers but would logically expect that provide the concentration of affimer is high ont he strip then the any virus at all will get caught

Ophidian

$100 a go to wait 48 hours for a test that probably is no better than any other PCR test - yep - that should corner the market.....

Ophidian

@cj62 - is that before they turn into killer bees ?

Lulu - you are funny. Sometimes it's hard to see the wood for the sleaze

remind me again who you were before you were Lulu ?

haha snap

it's the language gives it away isn't it

Is it just a coincidence that Solana (member since April 29th) pops up just as Lulu is totally expunged from LSE - (go check it out)

??

Ophidian

Matthew 7:6

@HullFC -to this point I've just ignored your rudeness. Bluntly - you're just not bright enough to bother about. Gloating - no - making sure you particularly as someone who ALWAYS tries to undermine or negate genuine and most often accurate information that you don't like are aware that it is possible to read the situation and project outcomes independently of an obviously over stretched position. You're desperate aren't you. You used to steal my posts and pass them off on Twitter as insight but you are incapable of seeing what is right in front of your nose and probably of understanding just how to interpret what is there for everyone to see and benefit from. Pretty much everything I have posted as been right when I've called it up or down I've done it based on a more nuanced interpretation of data than you obviously can get your tiny intellect around. You and your sidekick Lulu - how is the crack pot revelation theory this week by the way what's the current promise or insights for RNS news ? - you both insinuate I am short I am not and never have been short except when I RIGHTLY told you all I was opening a short position on SpreadBet to protect my long position while the share price fell according to my accurate prediction. I also told you when I closed it.

There are probably those who read this board who might like to hear real insight or perspective you however just drown the place out with your unceasing nonsense and drivel.

What gets you most ? the fact that I am pretty much always right ? that you are wrong ? or that you just can't work out how I can know and you still spin your wheels wondering what is going on ?

Well now we know - nothing more than an orchestrated move by the MMs. They have cleaned up today 3.74 million shares into their sweaty clutches. I don't like to say I told yo so but...……

And there is more to come.

Ophidian

so far it has worked like a charm for them...….

@Alma - MMs are exempt shorting rules they are allowed to naked short to some extent in the "making a market" role. They also do it when they have a request to sell and they know they will be getting the shares later to cover off their position. I have been wondering for sometime if they know they have a 10 million sell arriving once the share price reaches an agreed level to square their books.

Ophidian

Not sure I can illuminate any reason at all for todays move other than - I don't trust it ! Nothing very much has changed from a trade analysis perspective. Two very quiet days with little or no volume to speak of with a slowly eroding share price and even a slightly tightening spread - it all looked pretty much as I would have expected then today from nowhere on no volume as well a big move.

My only observation would be it happened after a Trade of 1 share and I'm sure you all know the folklore surrounding a one trade. Maybe the MMs are just playing games. They certainly still carry a significant short position.

If it can be held on to great news but I'm not convinced it's anything real.

Ophidian

O&W - with tests that look for an RNA fragment that might easily be the case but with the AFFIMER it's sort of Binary - either it grabs the virus by the spike - or it doesn't so it never grabs the wrong Virus and gives a false positive and so long as the viral load is above a minimum threshold so there are virus particles in the saliva to grab if they are there - it gets them. Voila.

Ophidian

Whilst a mechanism of action isn't difficult to imagine - this would constitute a new drug and would therefore be subject to a very long development / registration process compared to for example re-purposing an existing registered medicine. Sadly you can't just short cut or speed up tox studies and 3 phases of clinical trials.

Ophidian

@PAH00 - yes. From the Avacta website "Affimer® proteins can be genetically fused to each other to form multi-specific therapies, or to a variety of other proteins, such as the C-terminus of an antibody, to generate bispecific biobetter formats. Importantly, all formats of these multi-specific Affimer® therapeutics retain high expression yields. They can also be conjugated to toxins to form Affimer-drug conjugates."

Ophidian

Right now in spite an advertised ask of 1.5 you can buy at 1.3 I don't advocate missing our if you think news is inbound but if it isn't (and my feeling is that it is still too early given world events) then I think this will go back to where it was before in the 0.95 - 1.1 range so that MMs can balance their books (for the FOURTH time)

Ophidian

@montmuzard - exactly

Ophidian